Publikationen

2023

Koch Jonas, Scheps Daniel, Gunne Matthias, Boscheinen Oliver, Frech Christian
"Effect of salt modulators on the elution behavior of insulin and the separation of product-related impurities in reversed-phase chromatography"
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 1224: 123735; doi:10.1016/j.jchromb.2023.123735

Koch Jonas, Scheps Daniel, Gunne Matthias, Boscheinen Oliver, Frech Christian
"Mechanistic modeling of cation exchange chromatography scale-up considering packing inhomogeneities"
Journal of separation science; doi:10.1002/jssc.20200031

2022

Stange Carolin, Hafiz Supriyadi, Erwin Nelli, Hafner Mathias, Frech Christian
"Influence of excipients in Protein A chromatography and virus inactivation"
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 1214: 123563; doi:10.1016/j.jchromb.2022.123563

Seelinger Felix, Wittkopp Felix, von Hirschheydt Thomas, Frech Christian.
"Anti-Langmuir elution behavior of a bispecific monoclonal antibody in cation exchange chromatography: Mechanistic modeling using a pH-dependent Self-Association Steric Mass Action isotherm "
Journal of chromatography. A 1689:463730; doi:10.1016/j.chroma.2022.463730

Stange Carolin, Sánchez-Reyes Gabriela, Graalfs Heiner, Frech Christian
"Influence of ligand density variations on the two peak elution behavior of a monoclonal antibody in cation exchange chromatography"
Journal of chromatography. A 1676:463265; doi:10.1016/j.chroma.2022.463265

Seelinger Felix, Wittkopp Felix, von Hirschheydt Thomas, Hafner Mathias, Frech Christian
"Application of the Steric Mass Action formalism for modeling under high loading conditions:Part 1. Investigation of the influence of pH on the steric shielding factor"
Journal of chromatography. A 1676:463265; doi:10.1016/j.chroma.2022.463265

Seelinger Felix, Wittkopp Felix, von Hirschheydt Thomas, Frech Christian.
"Application of the Steric Mass Action formalism for modeling under high loading conditions:Part 2. Investigation of high loading and column overloading effects"
Journal of chromatography. A 1676:463266; doi:10.1016/j.chroma.2022.463266

Koch Jonas, Scheps Daniel, Gunne Matthias, Boscheinen Oliver, Hafner Mathias, Frech Christian
"Mechanistic modeling and simulation of a complex low and high elution behavior of a polypeptide in cation exchange chromatography"
Journal of separation science 45(12); doi:10.1002/jssc.202200098

2021

Stange Carolin, Hafiz Supriyadi, Korpus Christoph, Skudas Romas, Frech Christian
"Influence of excipients in Protein A chromatography and virus inactivation"
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 1179: 122848; doi:10.1016/j.jchromb.2021.122848

2020

Sánchez Reyes Gabriela, Graalfs Heiner, Hafner Mathias, Frech Christian
"Mechanistic modeling of ligand density variations on anion exchange chromatography"
Journal of Separation Science, 2020; doi: 10.1002/jssc.202001077

2018

Lee Yi Feng, Jöhnck Matthias, Frech Christian
"Evaluation of differences between dual salt-pH gradient elution and mono gradient elution using a thermodynamic model: Simultaneous separation of six monoclonal antibody charge and size variants on preparative-scale ion exchange chromatographic resin"
Biotechnology progress, 2018; doi: 10-1002/btpr2626

Wittkopp Felix, Peeck Lars, Hafner Mathias, Frech Christian
"Modeling and simulation of protein elution in linear pH and salt gradients on weak, strong and mixed cation exchange resins applying an extended Donnan ion exchange model"
Journal of chromatography A, 2018; doi:10.1016/j.chroma.2018.02.020

2017

Lee Yi Feng, Kluters Simon, Hillmann Mirjam, von Hirschheydt Thomas, Frech Christian
"Modeling of bispecific antibody elution in mixed-mode catin-exchange chromatography"
Journal of separation science, 2017; doi:10.1002/jssc.201700313.

2016

Lee Yi Feng, Graalfs Heiner, Frech Christian
"Thermodynamic modeling of protein retention in mixed-mode chromatography: An extended model for isocratic and dual gradient elution"
Journal of chromatography. A, 2016; doi:10.1016/j.chroma.2016.08.026

2015

Kluters Simon, Wittkopp Felix, Jöhnck Matthias, Frech Christian
"Application of linear pH gradients for the modeling of ion exchange chromatography: Separation of monoclonal antibody monomer from aggregates"
Journal of separation science; doi:10.1002/jssc.201500994

Kluters Simon, Frech Christian, von Hirschheydt Thomas, Schaubmar Andreas, Neumann Sebastian
"Solvent modulation strategy for superior antibody monomer/aggregate separation in cation exchange chromatography."
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 1006: 37-46; doi:10.1016/j.jchromb.2015.10.023

Kluters Simon, Hafner Mathias, von Hirschheydt Thomas, Frech Christian.
"Solvent modulated linear pH gradient elution for the purification of conventional and bispecific antibodies: Modeling and application"
Journal of chromatography. A; doi:10.1016/j.chroma.2015.09.053

Lee Yi Feng, Schmidt Michael, Graalfs Heiner, Hafner Mathias, Frech Christian
"Modeling of dual gradient elution in ion exchange and mixed-mode chromatography"
Journal of chromatography. A; doi:10.1016/j.chroma.2015.09.032

2014

Schmidt Michael, Hafner Mathias, Frech Christian
"Modeling of salt and pH gradient elution in ion-exchange chromatography"
Journal of separation science 37 (1-2): 5-13; doi:10.1002/jssc.201301007

2012

Kluters Simon, Neumann Sebastian, von Hirschheydt Thomas, Grossmann Adelbert, Schaubmar Andreas, Frech Christian.
"Mechanism of improved antibody aggregate separation in polyethylene glycol-modulated cation exchange chromatography"
Journal of separation science 35 (22): 3130-8; doi:10.1002/jssc.201200715

2011

Urmann Marina, Hafner Mathias, Frech Christian
"Influence of protein and stationary phase properties on protein-matrix-interaction in cation exchange chromatography"
Journal of chromatography. A 1218 (31): 5136-45; doi:10.1016/j.chroma.2011.05.085

2010

Potempa Marc, Hafner Mathias, Frech Christian
"Mechanism of gemini disulfide detergent mediated oxidative refolding of lysozyme in a new artificial chaperone system"
The protein journal 29 (7): 457-65; doi:10.1007/s10930-010-9279-8

Urmann Marina, Graalfs Heiner, Joehnck Matthias, Jacob Lothar R, Frech Christian
"Cation-exchange chromatography of monoclonal antibodies: Characterization of a novel stationary phase designed for production-scale purification"
mAbs 2 (4); doi:10.4161/mabs12303

2009

Bruch Thomas, Graalfs Heiner, Jacob Lothar, Frech Christian
"Influence of surface modification on protein retention in ion-exchange chromatography. Evaluation using different retention models"
Journal of chromatography 1216 (6): 919-26; doi:10.1016/j.chroma.2008.12.008